To cause to be estranged : to make unfriendly, hostile, or indifferent especially where attachment formerly existed. (Merriam – Webster)
To convey or transfer (something, such as property or a right) usually by a specific act rather than the due course of law. (Merriam – Webster)
To cause to be withdrawn or diverted. (Merriam – Webster)
Make (someone) feel isolated or estranged. (Oxford Dictionaries)
Make (someone) become unsympathetic or hostile. (Oxford Dictionaries)
If you alienate someone, you make them become unfriendly or unsympathetic towards you. (Collins Dictionary)
11 Ways to Lose Friends & Alienate People.
5 Ways to Alienate a Customer.
Don’t be a jerk: How not to alienate people abruptly, and risk losing everything they have to offer.